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KMID : 0377220030280010005
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Medical Journal of Chosun Univercity
2003 Volume.28 No. 1 p.5 ~ p.11
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Immunohistochemical labelling of the Interstitial Cells of Cajal(ICC) in the Mouse Gastrointestinal Tract
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Kim Young-Chul
Lim Gun-Han
Oh Jae-Wook
Chang In-Youb
Cha Kyung-Hun
Shin Moo-Kyoung
Kim Ju-Young
Yang Gyoung-Chul
Kim Jang-Man
Yang Gyoung-Chul
Park Do-Young
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Abstract
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Background and Objectives : Interstitial cells of Cajal(ICC) are the pacemakers in gastrointestinal tract that modulates gastrointestinal motiliey and these cells also transmit neural input from enteric nerves to smooth muscles. Recent work on tissues from patients with motility disorders that suggest that loss or defect in ICC could be related to pathophysiology in human and animal models. Immunolabelling of ICC in intestinal wall is recently developed by using specific marker, anti-c-kit antibody. Immunohistochemistry was done for ICC network in attempt to provide a morphological basis for the mechanism regulating gastrointestinal motility
Methods : Cryosection was done, and whole-mount preparations of mouse stomach, gastrointestinal tract were immunolabelled using the anti-c-Kit. Immunolabelled ICC networks were observed under a confocal laser scanning microscopy.
Results : According to three dimensional reconstruction study, we found that the c-Kit-positive celluar networks were widely distributed in the gastrointestinal muscle (1) circular muscle layer(IC-IM), (2) myenteric plexus(IC-MY), (3) deep muscular plexus(IC-DMP) in ileum, (4) submucosal plexus(IC-SMP) and longitudinal muscle layer(IC-LM) in colon.
Conclusion : The characteristic profiles of ICC celluar networks provide a morphological basis upon the mechanism regulating gastrointestinal motility. Additional studies for the enteric nerves-ICC interaction are need to evaluate the detailed roles of Icc in gastrointestinal tract.
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KEYWORD
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c-Kit, Interstitial cells of Cajal(ICC), Gastrointestinal motility, immunohistochemistry, Mouse
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